All efforts were made to minimize animal suffering and reduce the number of animals used. A recent epidemiological analysis in humans also found an association between diabetes prevalence and sugar availability [ 11 ].
Piche LA, Mahadevappa VG Modification of rat platelet fatty acid composition by dietary lipids of animal and vegetable origin. Coconut oil also contains a high proportion of saturated fatty acids. Figure 5. Besides medication, diet also plays an important role in the management of lipid and lipoprotein concentrations in blood.
Insulin resistance and excessive accumulation of lipids is strongly associated with non-alcoholic fatty liver disease NAFLDwhich represents the hepatic manifestation of a systemic impairment of the insulin network [ 14 ].
Specimens were then cleared in xylol, embedded in paraffin, sectioned at microns thickness and stained with hemtoxylin and eosin stain for examination described by Carleton.
Group 1: FEBS Lett J Biol Chem The increase in intensity of insulin in pre diabetes rat pancreatic islets is possibly because of increased demand of insulin by the body to achieve normoglycemia for which per cell synthesis of insulin is increased leading to intense staining.
Tissue oxidative stress The oxidative fluorescent dye dihydroethidine DHE was used to evaluate in situ formation of ROS according to a method described by Dal-Ros et al. At the beginning of the study, 5 rats were sacrificed Ctr-rats, M0. High-fat diet-induced obesity Rat model: Fructose is used commercially as a sweetening substitute fructose corn syrup for glucose or sucrose, in the preparation of desserts, condiments, and carbonated beverages [ 6 ].
However, the cellular and molecular mechanism s underlying these basic defects is not clear yet. After 3 weeks of study, pioglitazone at a dose of 2. Large clinical studies of both forms of diabetes have demonstrated that hyperglycaemia plays an important role in the pathogenesis of microvascular complications [ 23 ].
Figure 7. In situ liver macrophages As previously described by Dal S et al. The consumption of large amounts of added sugar, a prominent source of low-nutrient calories in processed or prepared foods and caloric beverages i. CA, USA. High fructose consumption increases the likelihood of weight gain and reduces circulating leptin concentration leading to IR associated with hyperinsuliemia, hypertriglyceridemia and hyperglycemia.
· Fig. 1. Differing propensity towards HFD-induced body weight gain. (A) Phenotypic appearance of chow-fed Wistar rats compared with HFD-fed and partially DIO resistant (DIOres) or DIO sensitive (DIOsens) rats (Perez-Tilve et al., ). Rat models including outbred SpragueDawley and Wistar rats are popular strains to study obesity as they readily gain weight on high-fat diets.
In particular, Sprague-Dawley rats have been studied. humanized rat model, impaired glucose tolerance, prediabetes, high fat diet, Glut4 mRNA, insulin immunohistochemistry Introduction Diabetes is a chronic disease and for.
I made high-fat diet consisting of rat chow diet, corn (maize) oil, ghee butter, milk powder, and refined brown sugar powder. Apparently, the samples didn't fancy the food and nearly ate nothing. · High-fat diet-induced obesity Rat model: a comparison between Wistar and Sprague-Dawley Rat.
Marques C(1), Meireles M(2), Norberto S(2), Leite J(2), Freitas J(3), Pestana D(1), Faria A(4), Calhau C(1).Cited by: Angéloco, et al. 2 used bioelectrical impedance analysis in rats fed diets - high in fat and sucrose - and correlated with the analysis of biochemical and anthropometric parameters.
The bioelectrical impedance was not sensitive enough to detect changes in body composition; however, anthropometric parameters - body mass index, index of Lee and circumference of the abdomen - can estimate body.